5th Functional DNA Nanotechnology Workshop, Rome 25-27 May 2022


Below is reported the final program. The book of abstract of the workshop can be found at this link (here).


Wednesday 25th May:

10:30- 12:00           Registration

12:00 – 12:10           Opening

12:10 – 12:40           Invited Lecture (IL) 1: Hao Yan “Designer Nucleic Acid Architectures for Programmable Self-assembly”, Arizona State University, USA

12:40 – 12:55           O1: Enzo Kopperger “Engineering Electrically Driven DNA-Based Mechanisms” Technical University of Munich, Germany

12:55 – 13:10           O2: Anton Kuzyk, “DNA-origami-based plasmonic assemblies with tailored stimuli and optical responses”, Aalto University, Finland

13:10 – 13:25           O3: Minke A.D. Nijenhuis, “Folding dsDNA using Triplex Forming Oligonucleotides”, Aarhus University, Denmark

13:25 – 14:25           Refreshments + poster session

14:25 – 14:55           IL2: Laura Na Liu, “Dynamic plasmonic systems with controlled motion on the nanoscale”, University of Stuttgart, Germany

14:55 – 15:10           O4: Haggai Shapira, “Development of a High-performance DNA Origami Rotary Motor, Monitored by Defocused Imaging of Gold Nanorods”,       Ben Gurion University of the Negev, Israel

15:10 – 15:25           O5: Damien Baigl, “Isothermal self-assembly of multicomponent and evolutive DNA nanostructures”, Ecole Normale Supérieure (ENS), France

15:25- 15:40           O6: Iris Seitz, “Optically responsive protein coating of DNA origami for antigen targeting”, Aalto University, Finland

15:40 – 15:50          Flash presentations (2 minutes each x 5)

15:50 – 16:30          Tea Break + poster session

16:30 – 17:00           IL3: Maartje M.C. Bastings, “Patterns in Biology: DNA-origami as nano-tool to control multivalent binding”, École Polytechnique Fédérale de Lausanne (EPFL), Switzerland

17:00 – 17:15           O7: Claudia Corti, “Hybrid gold-DNA origami nanostructures for colorimetric sensing”,  Institut Langevin – ESPCI-PSL , France

17:15 – 17:30           O8: Gregor Posnjak, “DNA-origami based diamond type lattice with visible wavelength periodicity”, LMU Munich, Germany

17:30 – 17:45           O9: Casey M. Platnic, “A dissipative pathway for the structural evolution of DNA fibres”, University of Cambridge, UK

17:45 – 18:00           O10: Alan Szalai, “Orientation of dsDNA relative to graphene determined by single-molecule fluorescence lifetime microscopy”, Ludwig-Maximilians-Universität München (LMU), Germany


Thursday 26th May:

9:00 – 9:30                IL4: Itamar Willner, “Aptananozymes – A New Class of Aptamer-Modified Nanoparticles for Catalysis and Chemodynamic Medicine”, The Hebrew University of Jerusalem, Israel

9:30 – 9:45                O11: Amelie Heuer-Jungemann, “New insights into the DNA origami silicification reaction mechanism by in situ small angle X-ray scattering”, Max Planck Institute of Biochemistry, Martinsried, Germany

9:45 – 10:00             O12: Joel Spratt, “Tuning the insulin receptor signalling pathway response using insulin-DNA origami nanostructures”, Karolinska Institute Stockholm, Sweden

10:00 – 10:15           O13: Felix J. Rizzuto, “DNA sequence and length dictate the assembly of nucleic acid block copolymers”, School of Chemistry, University of New South Wales, Australia

10:15 – 10:30           O14: Michael Pinner, “A DNA-based artificial membrane budding system”, Technical University of Munich, Germany

10:30 – 10:45           Flash presentations (2 minutes each x 5)

10:45 – 11:40           Coffee break + poster session

11:40 – 12:10           IL5: Lorenzo Di Michele, “A three-agent communication pathway triggered by bacterial metabolism (that uses DNA nanotechnology)”, Imperial College London, UK

12:10 – 12:25           O15: Yongzheng Xing, “Designer DNA-based Membrane Nanopores for Portable Sensing of Diagnostic Proteins”, University College London, UK

12:25 – 12:40           O16: Barbara            Saccà,  “Thermodynamic and kinetic properties of DNA-confined enzymes”, University Duisburg-Essen, Essen, Germany

12:40 – 12:55           O17: Adrian Leathers, “Reaction-diffusion patterning of DNA-based artificial cells”, University of Cambridge, UK

12:55 – 13:10           O18: Juliette Bucci, “Temporal control of DNA strand displacement reaction”, University of Rome, Tor Vergata, Italy


13:10 – 14:10           Lunch


14:10 – 14:40            IL6: Elisa Franco, “Dynamic control of DNA condensates via strand displacement” University of California, Los Angeles, USA

14:40 – 14:55           O19: Alexander J. Speakman, “Electrically Directed Gene Expression (EDGE): using switchable DNA triplexes and electrolysis to modulate transcription in a cell-free medium”, University of Edinburgh, UK

14:55 – 15:10           O20: Aleksandra Adamczyk, “Orienting single molecules in DNA origami constructs”, University of Fribourg, Switzerland

15:10 – 15:25           Flash presentations (2 minutes each x 5)

15:25 – 15:40           Irene Ponzo, “switchSENSE and proFIRE – a DNA-based technology to discover molecular interactions and preparation of pure protein-DNA conjugates”, Dynamic Biosensor, Germany

15:40 – 16:40           Coffee Break + informal discussion

16:40 – 20:00           Social programme (tour to Nemi – lake and town)

20:00                        Social dinner + Award Ceremony


Friday 27th of May


9:00 – 9:30       IL7: Andreas Walther, “Metabolic DNA Systems Inspired from Life:

Protocells and Systems with Lifecycles”, University of Mainz, Germany


9:30 – 9:45         O21: Matteo Castronovo, “Enzymatic DNA ligation within two-dimensional DNA origami depends on nanostructure shape”, University of Leeds, UK

9:45 – 10:00        O22: Alexis Vallée-Bélisle, “Bio-inspired DNA switches for sensing and drug delivery applications”, University of Montreal, Canada

10:00 – 10:15         O23: Giovanni Nava, “Probing the conformational dynamics of long unstructured single stranded DNA chains”, University of Milan, Italy

10:15 – 10:30           O24: Ioanna Smyrlaki, “DNA Origami nano-patterns as a Precise Tool to study clustering of Notch receptor” Karolinska Institute, Sweden

10:30 – 10:45           O25: Guillaume Gines, “DNA-enzyme neural networks enabling nonlinear concentration profile classification”, Gulliver Laboratory,Université Paris Sciences et Lettres, France


10:45 – 11:30           Coffee break


11:30 – 11:45           O26: Christoph Wälti, “Counting individual molecules: DNA nanostructures for diagnostic applications”, University of Leeds, UK

11:45 – 12:00           O27: Adrian Keller,  “Hierarchical self-assembly of DNA origami lattices at solid-liquid interfaces”, Paderborn University, Germany

12:00 – 12:15           O28: Rakesh Mukherjee, “Kinetic proofreading in a DNA strand displacement network”, Imperial College London, UK


12:15- 12:45            Award presentations + closing remarks


13:00                                    Light lunch






P-1:       Adam Dorey, University College London, “Synthetic protein-conductive membrane nanopores built with DNA”          .

P-2:       Aleksandra Bednarz, Aarhus University, “Ion-dependent structural integrity and reconfigurability of DNA origami nanostructures.

P-3:       Alessandro Cecconello, University of Padova, “Regulating in vitro transcription using RNA/DNA triplex-based biosynthetic machineries”.

P-4:       Alexander M. Kloosterman, Karolinska Institutet, “Spatial inference of barcoded transcripts from sequencing data”.

P-5:       Alexia Rottensteiner, University College London (UCL), “A Light-Actuated DNA Channel for Controlled Transport Across Membranes”.

P-6:       Ali Khoshouei, Technical University Munich, “CryoEM structure determination using DNA nanotechnology”.

P-7:       Ana Martins, University of Porto, “Neuronal targeting with functionalized tetrahedral DNA nanostructures”.

P-8:       Andreas Peil, University of Stuttgart; Max Planck Institute for Solid State Research, “DNA Assembly of Modular Components into a Rotary Nanodevice”.

P-9:       Andrew Stannard, Imperial College London, “Mechanically-modulated toehold mediated strand displacement“.

P-10:     Annelies Dillen, University of Leuven, “Duplexed aptamers on fiber optic surface plasmon resonance sensors: a winning combination for continuous biosensing”.

P-11:     Bhanu Kiran Pothineni, Paderborn University, “Novel vancomycin-conjugated DNA origami-based nanoantibiotics”            .

P-12:     Chalmers Chau, University of Leeds, “Single biomarker detection with affimer conjugated DNA origami through solid-state nanopore”.

P-13:     Coline Kieffer, Université Paris Sciences et Lettres, “Tunable-gain amplifier in DNA-enzyme reaction circuits and its applications in microRNA biosensing”.

P-14:     Wooli Bae, University of Surrey, “Building an RNA-Based Toggle Switch Using Inhibitory RNA Aptamers”.

P-15:     Christoph Pauer, Ludwig-Maximilians-Universität München (LMU), “Propulsion of magnetic beads asymmetrically covered with DNA Origami appendages”.

P-16:     Volodymyr Mykhailiuk, Technical University Munich, “DNAzymes for mass production of DNA oligonucleotides”.

P-17:     Christopher Frank, Technical University Munich, “Cell surface-mediated conformational changes of DNA-Origami objects“.

P-18:     Diana Morzy, EPFL, Switzerland, “Valency and entropic costs determine the cation-mediated DNA/lipid binding”.

P-19:     Elena-Marie Willner, Technical University Munich, “Virus neutralization using icosahedral DNA origami shells”.

P-20:     Elija Feigl, Technical University Munich, “WaffleCraft: Fully Automated Blocky DNA Origami Design Tool”.

P-21:     Fabian Kohler, Technical University of Munich, “Precision Design and Characterization of DNA Origami Corner Motifs using Cryo-EM”.

P-22:     Farah El Fakih, Ecole Normale Supérieure, “Reversible Supra‐Folding of User‐Programmed Functional DNA Nanostructures on Fuzzy Cationic Substrates”.

P-23:     Florian Rothfischer, Technical University of Munich/ Ludwig-Maximilians-Universität München, “Control of enzyme activity by a DNA nanoscale robotic arm”.

P-24:     Francesca Smith, Imperial College London, “Characterisation of RNA/DNA hybrid strand displacement kinetics”.

P-25:     Gerrit          Wilkens, Jagiellonian University, Malopolska Centre of Biotechnology, “Blowing “bubbles” with DNA origami”.

P-26:     Giacomo Fabrini, Imperial College London, “Cation-Responsive and Photocleavable Hydrogels from Noncanonical Amphiphilic DNA Nanostructure”.

P-27: Viktorija Kozina, Technical University Munich, “Targeting antigen patterns with programmable T-cell engagers”.

P-28:     Igor Baars, Karolinska Institutet, “Spatial reconstruction using barcoded DNA sequences”.

P-29:     Jacky Loo, Aalto University, “Colorimetric Visualization with Visible Chirality”.

P-30:     Jing Huang, CENIDE and ZMB, University of Duisburg-Essen, “A DNA-confined unfoldase/protease nanomachine”.

P-31:     Viktorija Glembockyte, LMU Munich, “Self-regeneration and self-healing in DNA nanostructures”.

P-32:     Nada Farag, University of Rome Tor Vergata, “Programmable decoration of DNA-based scaffold through dynamic exchange of structural motifs”.

P-33:     Kevin Jahnke, Max Planck Institute for Medical Research; Heidelberg University, Rational engineering of DNA cytoskeletons for synthetic cells”.

P-34:     Lena Stenke, University Duisburg-Essen, Germany, “Dynamics of DNA origami filaments growth from a ditopic monomer”.

P-35:     Lorena Baranda, University of Rome Tor Vergata, “Protein-Templated Reactions Using DNA-Antibody Conjugates”.

P-36:     Ulrich          Kemper, University of Leipzig, “DNA mold-based fabrication of palladium nanostructures”.

P-37:     Ludwig Rotsen, Univ. Grenoble Alpes, “Substrate-assisted self-assembly of DNA origamis for lithographic applications”.

P-38:     James Vesenka, University of New England and Leibniz-IPHT Biophotonics, “AFM analysis of G-wire DNA structure and nanoparticle decoration”.

P-39:     Marcel Hanke, Paderborn University, “Salting-out of DNA Origami Nanostructures by Ammonium Sulfate”.

P-40:     Tania Patino, University of Rome Tor Vergata, “Bioengineering DNA-based enzyme-powered nanoswimmers”.

P-41:     Sara Bracaglia, University of Rome Tor Vergata, “Programmable cell-free transcriptional switches for antibodies detection”.

P-42:     Marcus Fletcher, University of Cambridge, “G-Quadruplex DNA based fluorescent sensing for quantification of potassium ion flux across giant proteoliposomes”.

P-43:     Matthew Aquilina, University of Edinburgh, “Multiplexed Label-Free Biomarker Detection by Targeted Disassembly of Variable-Length DNA Payload Chains”.

P-44:     Maximilian Nicolas Honemann, Technical University of Munich, “A novel lattice design for scaffolded DNA origami structures”.

P-45:     Michal Walczak, University of Cambridge, “Stimuli-responsive DNA particles underpin three-agent signaling networks with live bacteria and synthetic cells”.

P-46:     Daniela Sorrentino, University of Rome Tor Vergata, “Allosteric regulation of DNA-based nanodevices using in vitro transcription”.

P-47:     Nathanv Wu, University of Edinburgh, “A DNA Nanotechnology Assay to Detect Double-Stranded DNA for Medical Applications”.

P-48:     Nico Alleva, Max-Planck Institute for Polymer Research, “Diverse, highly efficient grafting to strategy for the patterning of DNA-origami”.

P-49:     Richard Kosinski, University of Duisburg-Essen, “The role of DNA nanostructures in the catalytic properties of an allosterically regulated protease”.

P-50:     Roger Rubio Sanchez, Imperial College London, “A modular, dynamic, DNA-based platform for regulating cargo distribution and transport between lipid domains”.

P-51:     Sabrina Gambietz, University Duisburg-Essen, Germany, “Thermal and mechanical properties of topologically identical origami domains at the ensemble and single-molecule level”.

P-52:     Sayantan De, University Duisburg-Essen, Essen (Germany), “A DNA logic gate to sense molecular distances”.

P-53:     Seppe Driesen, University of Leuven, “Towards DNA-only digital biosensing with DNA nanosensors”.

P-54:     Sergii Rudiuk, Ecole Normale Supérieure, “DNA-protein nanogels as transfectable multienzymatic nanoreactors“.

P-55:     Sofia Julin, Aalto University, “pH-Responsive DNA Origami Lattice”.

P-56:     Steffan Møller Sønderskov, Aarhus University, “High-resolution surface charge density visualization of DNA nanostructures”.

P-57:     Serena Gentile, University of Rome Tor Vergata, “Spontaneous reorganization of DNA-based polymers in higher ordered structures fueled by RNA”.

P-58:     Teun Huijben, Technical University of Denmark, “Fast and exact reduction of mislocalizations near spherical nanoparticles by a fully analytical PSF”.

P-59:     Miguel Paez-Perez, Imperial College London, “Effect of lipid composition on the efficiency of fusogenic DNA nanostructures”.

P-60:     Ken Sachenbacher, Technical University Munich, “Triple-stranded DNA as a structural element in DNA origami”.

P-61:     Marco Lolaico, Karolinska Institute, “Enhanced stiffness of wireframe DNA nanostructures with square lattice edges”.

P-62:     Neda Bagheri, University of Rome Tor Vergata, “Enhancement of CRISPR/Cas12a trans-cleavage Activity Using Hairpin DNA Reporters”.